Overview
The high morbidity and mortality of African swine fever (ASF)
has a severe impact on the global swine industry.
Features &
Benefits
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- Swine-specific blocking reagent
- Provides swine-specific blocking reagent that efficiently filters out repetitive sequences, allowing for the selective retrieval of ASFV sequences
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- Comprehensive analysis of ASFV subtypes
- Detect genotype II virus subtypes with specifically designed probes
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- Convenient testing
- Highly accurate results from blood sample, that is considered more
challenging due to its lower viral load compared to concentrated
culture supernatant or spleen tissue sample
Workflow
Celemics’ African Swine Fever Virus Panel uses DNA samples obtained from swine peripheral blood which contains a low amount of viral DNA compared
to other tissue samples such as the spleen. Because blood samples are easier to obtain compared to other sample types, this panel provides convenience
in the experimental preparation stage. Also, target capture probes specialized for ASFV DNA provide sufficient virus sequence information,
leading to a cost-effective experimental experience by preventing additional NGS operation.
Performance
- Advanced target enrichment technology enabling
exceptional capture performance with high
coverage and uniformity - The panel validation result shows high uniformity
and high coverage at all levels
- Outstanding target capture performance
to cost-effective research and virus detection -
With the same sequencing amount, target enrichment (TE)
NGS yielded 29% virus reads out of a total of 1,039,018 reads
while whole genome sequencing (WGS) yielded 0.5%
virus reads (green) out of a total of 39,261 reads.
Applications
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Research and detection of ASFV virus
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Investigation of route of ASFV infection