Double Stranded cDNA Synthesis Kit

Stabilize RNA to double-stranded cDNA for unquestionable performance

Overview

Double-Stranded cDNA Synthesis Kit

Celemics’ double stranded cDNA synthesis kit is designed to provide the optimized for template for NGS-based RNA sequencing.


The kit includes all components from RNA fragmentation to double stranded cDNA synthesis for NGS library preparation. The undeniable stability and performance of the kit allows for the cDNA synthesis even from low amounts of RNA samples with high accuracy and reduced reaction time.

Library Preparation Kit

cDNA Synthesis Workflow

  • Sample amount : 25 ng to 1 μg (as low as 10 ng)*
  • Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
  • * Carrier RNA is required for sample amount < 25 ng
cDNA Synthesis Workflow

RNA Fragmentation

1st. Strand Synthesis

2nd. Strand Synthesis-1

2nd. Strand Synthesis-2

Double-stranded cDNA

of an RNA sample

cDNA Synthesis Workflow

  • Sample amount : 25 ng to 1 μg (as low as 10 ng)*
  • Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
  • * Carrier RNA is required for sample amount < 25 ng
cDNA Synthesis Workflow

RNA Fragmentation

1st. Strand Synthesis

2nd. Strand Synthesis-1

2nd. Strand Synthesis-2

Double-stranded cDNA

of an RNA sample

cDNA Synthesis Workflow

  • Sample amount : 25 ng to 1 μg (as low as 10 ng)*
  • Assay time : 30 minutes for RNA fragmentation and 2 hours for strand synthesis
  • * Carrier RNA is required for sample amount < 25 ng
cDNA Synthesis Workflow

RNA Fragmentation

1st. Strand Synthesis

2nd. Strand Synthesis-1

Double-stranded cDNA
of an RNA sample

2nd. Strand Synthesis-2

Double-stranded cDNA
of an RNA sample

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Resources

Technical Resources

[Product Overview] Celemics Accessories

[Product Overview] Celemics Target Enrichment Panel Overview

[Catalogue] Celemics Products & Service Catalogue_All Products & Service

Safety Data Sheets

MSDS_Double-Stranded cDNA Synthesis