Methylation Sequencing Panel

Facilitate Epigenetics research with Celemics’ NGS solution

Overview

Methylation Sequencing

Methylation sequencing can identify the locations and levels of methylated DNA in a genome-wide or targeted manner for various applications of epigenetic research. Celemics’ solution for DNA methylation sequencing can provide more comprehensive and detailed patterns of either genome-wide or specific target region of your choice.

Standard Workflow of Targeted Methyl-seq

Methylation Sequencing

Standard Workflow of Targeted Methyl-seq

Standard Workflow of Targeted Methyl-seq

Standard Workflow of Targeted Methyl-seq

DNA

Sample

Methylated 

adaptor

On-bead

conversion

protocol

Index PCR

Post-PCR

Library

Preparation

Bisulfite

Conversion

Target

Enrichment

Sequencing

Targeted

Methyl-seq Data

Celemics Proprietary Probe Design for Methyl-seq

Celemics Proprietary Probe Design for Methyl-seq

Celemics Proprietary Probe Design for Methyl-seq

“G/A” panel

“C/T” panel

Pre-PCR

Bisulfite

conversion

Celemics Proprietary Probe Design for Methyl-seq

Features & Benefits

Probe Specifically Designed for Methyl-seq

Celemics introduces elaborate design considering the sequence alteration by bisulfite conversion. We perform comparison analysis of the sequences before and after bisulfite conversion, enabling accurate detection of methylation sites.

BI analysis for methylation sequencing

BI analysis for methylation sequencing

BI analysis for methylation sequencing

Alignment

FASTQ Filter

Methylation

Extractor

Visualization

Methylation

Pattern

Deduplication

Raw

Data

Bismark

Standard Analysis Pipeline Targeted Methyl-seq

Compatible with Various Sample Types and C/T Conversion Method

Along with proprietary probes and exclusive workflow for methyl-seq, this panel allows methylation analysis from various sample sources.

As method for bisulfite conversion preference can be diverse, we also offer a market leading quality of panel that is compatible with both enzyme or chemical C/T conversion method; no compromised sequencing data depending on the conversion method

High Reproducibilty of Methylation Pattern Analysis

The results demonstrate high reproducibility of the analysis, yielding the same methylation patterns when repeatedly tested with the identical specimens.

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Incomparable quality magnetic bead for simple, flexible, and reproducible purification

Customized NGS Panel

Customization to Next Level; tailored NGS panel customization and assay optimization

Resources

Technical Resources

[Product Overview] Targeted Methylation Sequencing Panel

[Product Overview] Celemics Target Enrichment Panel Overview

[Catalogue] Celemics Products & Service Catalogue_All Products & Service

Safety Data Sheets

MSDS_Methylation Sequencing Panel_Illumina_Enzymeplus

MSDS_Methylation Sequencing Panel_Illumina

MSDS_Methylation Sequencing Panel_Thermo Fisher

MSDS_Methylation Sequencing Panel_MGI_Enzymeplus

MSDS_Methylation Sequencing Panel_MGI

References

Epigenomics

Identification of tissue of origin in cancer of unknown primary using a targeted bisulfite sequencing panel

Bae JM, Ahn JY, Lee H, Jang H, Han H, Jeong J, et al. Identification of tissue of origin in cancer of unknown primary using a targeted bisulfite sequencing panel. Epigenomics. 2022 May;14(10):615–28.

 

10.2217/epi-2021-0477


View Detail >

Investig Clin Urol

Germline pathogenic variants in unselected Korean men with prostate cancer

So MK, Ahn HK, Huh J, Kim KH. Germline pathogenic variants in unselected Korean men with prostate cancer. Investig Clin Urol. 2022 May;63(3):294–300.

 

DOI 10.4111/icu.20220044


View Detail >

Scientific Reports

Feasibility of targeted cascade genetic testing in the family members of BRCA1/2 gene pathogenic variant/likely pathogenic variant carriers

Lee J, Ham JY, Park HY, Jung JH, Kim WW, Kang B, et al. Feasibility of targeted cascade genetic testing in the family members of BRCA1/2 gene pathogenic variant/likely pathogenic variant carriers. Sci Rep. 2022 Feb 3;12(1):1842.

 

DOI 10.1038/s41598-022-05931-3


View Detail >

BMC Medical Genomics

A novel bi-alleleic DDX41 mutations in B-cell lymphoblastic leukemia: case report

Shin WY, Yoon SY, Park R, Kim JA, Song HH, Bang HI, et al. A novel bi-alleleic DDX41 mutations in B-cell lymphoblastic leukemia: case report. BMC Med Genomics. 2022 Mar 4;15(1):46.

 

DOI 10.1186/s12920-022-01191-2


View Detail >